KMID : 0379520040200010031
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Çѱ¹µ¶¼ºÇÐȸÁö 2004 Volume.20 No. 1 p.31 ~ p.36
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Effect of Radiation on mRNA Expression of Ceruloplasmin Gene
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Oh Youn-Kyoung
Lim Hee-Young Kim Jong-Soo Yun Choong-Hyo Kim In-Gyu Yoon Byoung-Su
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Abstract
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Against environmental stress, ceruloplasmin which is a plasma protein, are believed to play central roles in antioxidant- or peroxidase-activity in blood stream to remove free radicals, which may be caused by exposing of gamma-irradiation. In human U-937 cells exposed to gamma-irradiation, the levels of mRNA in ceruloplasmin gene were measured on 0, 4, 12, 24 hr after exposing by using comparative RT-PCR (Reverse transcriptase-polymerase chain reaction) which was achieved to compare with house keeping genes such as beta-actin and hprt. After gamma-irradiation of 100 rads or 200 rads, the total quantities of RNA were increased as dose and time dependent manner. On the contrary, the variation of mRNA expression in ceruloplasmin was not found until 4 hr after irradiation. After 12 hr and 24 hr of irradiation, the levels of mRNA in ceruloplasmin were significantly increased as dose and time dependent manner than un-exposed cells.
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KEYWORD
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Ceruloplasmin, gamma-irradiation, U-937 cell, RT-PCR.
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